https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Role of iron in the pathogenesis of respiratory disease https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31006 Wed 02 Mar 2022 14:28:48 AEDT ]]> The fibrogenic actions of the coagulant and plasminogen activation systems in pulmonary fibrosis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47646 Tue 24 Jan 2023 14:51:39 AEDT ]]> Factors affecting cytotrophoblast cell viability and differentiation: evidence of a link between syncytialisation and apoptosis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:12438 Sat 24 Mar 2018 08:15:27 AEDT ]]> Transcriptional regulation and functional implication of the grass carp CITED1 (gcCITED1) in the negative regulation of HIF-1 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10574 Sat 24 Mar 2018 08:10:44 AEDT ]]> Amyloid β induces microglia to phagocytose neurons via activation of protein kinase Cs and NADPH oxidase https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:25871 Sat 24 Mar 2018 07:25:52 AEDT ]]> Disruption of ß-catenin/CBP signaling inhibits human airway epithelial-mesenchymal transition and repair https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22016 Sat 24 Mar 2018 07:15:45 AEDT ]]> Selective modulation of different GABA<sub>A</sub> receptor isoforms by diazepam and etomidate in hippocampal neurons https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23577 A (γGABAA) receptors increases channel conductance by facilitating protein interactions involving the γ2-subunit amphipathic (MA) region, which is found in the cytoplasmic loop between transmembrane domains 3 and 4 (Everitt et al., 2009). However, many drugs, predicted to act on different GABAA receptor subtypes, increase channel conductance leading us to hypothesize that conductance variation in GABAA receptors may be a general property, mediated by protein interactions involving the cytoplasmic MA stretch of amino acids. In this study we have tested this hypothesis by potentiating extrasynaptic GABAA currents with etomidate and examining the ability of peptides mimicking either the γ2- or δ-subunit MA region to affect conductance. In inside-out hippocampal patches from newborn rats the general anesthetic etomidate potentiated GABA currents, producing either an increase in open probability and single-channel conductance or an increase in open probability, as described previously (Seymour et al., 2009). In patches displaying high conductance channels application of a δ(392-422) MA peptide, but not a scrambled version or the equivalent γ2(381-403) MA peptide, reduced the potentiating effects of etomidate, significantly reducing single-channel conductance. In contrast, when GABA currents were potentiated by the γ2-specific drug diazepam the δ MA peptide had no effect. These data reveal that diazepam and etomidate potentiate different extrasynaptic GABAA receptor subtypes but both drugs modulate conductance similarly. One interpretation of the data is that these drugs elicit potentiation through protein interactions and that the MA peptides compete with these interactions to disrupt this process.]]> Sat 24 Mar 2018 07:12:45 AEDT ]]> Extracellular matrix-mediated regulation of cancer stem cells and chemoresistance https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47881 Fri 03 Feb 2023 15:52:53 AEDT ]]> TLR2 ligation induces corticosteroid insensitivity in A549 lung epithelial cells: anti-inflammatory impact of PP2A activators https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:25080 Fri 01 Apr 2022 09:27:01 AEDT ]]>